Chemical composition and biological activity of essential oil of Achyrocline ramosissima Britton ex Rusby (Asteraceae)

  • Diolimar Buitrago
  • Antonio Morales
  • Luis Rojas-Fermín
  • María Lucena
  • Liliana Araujo
  • Laila M Moujir
Palabras clave: Achyrocline ramosissima, essential oil, antibacterial activity, cytotoxic activity


Essential oil from fresh aerial parts of Achyrocline ramosissima Britton ex Rusby (Asteraceae) collected in the Venezuelan Andes was obtained by hydrodistillation and analyzed by GC/MS. A yield of 0.10% w/v was afforded, and thirty seven components were identified by comparison of their retention indices (RI) and mass spectra with the Wiley GC-MS Library Data. The major components identified were rosifoliol (31.69%), β-caryophyllene (8.61%), guaiol (3.95%), β-eudesmol (3.33%) and selina-3,7 (11)-diene (2.99%). Antimicrobial activity of A. ramosissima essential oil was also evaluated against Gram positive, Gram negative bacterial strains and Candida albicans yeast. The results showed that, this oil is effective against Gram positive bacteria Staphylococcus aureus ATCC 6538 with MIC values ranging from 50 to 100 μg/mL and MBC values > 200 μg/mL as well as Bacillus subtilis CECT 39 with MIC values of 50 μg/mL and MBC of 100 μg/mL, however a low activity was observed against Gram negative bacterial strains, Pseudomonas aeruginosa AK 958, Escherichia coli CECT 99 and C. albicans yeast performing MIC and MBC values > 200 μg/mL. Cytotoxic activity was also determined against HeLa (cervix carcinoma), A-459 (lung carcinoma), MCF-7 (breast adenocarcinoma) human cancer cell lines and against normal Vero cells (African green monkey kidney), exhibiting antiproliferative effects with IC50 values of 28.2 μg/mL (HeLa cells). This is the first report regarding the chemical composition, antibacterial and cytotoxic activities of the essential oil from this species.


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