Pedalitin from Isodon japonica, an inactivation of soybean lipoxygenase-1

  • Tae Joung Ha
  • Takahiko Isobe
  • Enrique Werner
  • Julio Alarcon
  • Isao Kubo
  • Carlos L. Cespedes
Palabras clave: Rabdosia japonica, Pedalitin, Lipoxygenases inhibitor

Resumen

Pedalitin, isolated from the aerial part of Rabdosia japonica (Labiatae), inhibited soybean lipoxygenase-1 (EC 1.13.11.12, Type I) with an IC50 of 152.5 M. The progress curves for an enzyme reaction, pedalitin inactivate the lipoxygenase-1 in a time dependent, irreversible manner, exhibiting kinetics with a kinact/KI of 59.6 ± 10 mM-1min-1. In the pseudoperoxidase activity, pedalitin is very slowly oxidized by the soybean lipoxygenase-1 catalyzed decomposition of lipid hydroperoxides.

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